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glutathione sepharose 4b  (Cytiva Europe)
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Cytiva Europe glutathione sepharose 4b
Glutathione Sepharose 4b, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/cytiva+europe___17075601?v=Cytiva+Europe
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glutathione sepharose 4b - by Bioz Stars, 2026-07
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e18 gst ncegt1  (Kaneka Corp)
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Kaneka Corp e18 gst ncegt1
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
E18 Gst Ncegt1, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/pmc13273134-159-21-8?v=Kaneka+Corp
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e18 gst ncegt1 - by Bioz Stars, 2026-07
86/100 stars
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gst  (Kaneka Corp)
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Kaneka Corp gst
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
Gst, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/pmc12967149-229-5-7?v=Kaneka+Corp
Average 86 stars, based on 1 article reviews
gst - by Bioz Stars, 2026-07
86/100 stars
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gst resin  (Brickell Biotech)
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Brickell Biotech gst resin
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
Gst Resin, supplied by Brickell Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/pm42241470-276-14-17?v=Brickell+Biotech
Average 86 stars, based on 1 article reviews
gst resin - by Bioz Stars, 2026-07
86/100 stars
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anti gst  (Abmart Inc)
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Abmart Inc anti gst
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
Anti Gst, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/pm42277594-145-3-6?v=Abmart+Inc
Average 86 stars, based on 1 article reviews
anti gst - by Bioz Stars, 2026-07
86/100 stars
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gst tag  (Covance)
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Covance gst tag
Effects of solubility tags and stability mutations on <t>NcEGT1</t> expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 <t>kDa),</t> <t>GST-NcEGT1</t> (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.
Gst Tag, supplied by Covance, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/pm42248824-77-3-0?v=Covance
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gst tag - by Bioz Stars, 2026-07
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gst 4 detoxification  (Proteostasis Therapeutics)
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Proteostasis Therapeutics gst 4 detoxification
Effect of EA on anti-stress genes expression level. (A,B) EA significantly induced the expression of SOD-3::GFP. (C,D) EA significantly induced the expression of <t>GST-4::GFP.</t> (E,F) EA significantly induced the expression of HSP-16. 2::GFP after incubation at 37 °C for 30 min. (G) EA significantly activated the mRNA expression level of sod-3, gst-4 and hsp-16.2. The images were analyzed with ImageJ software and numerical data were analyzed by Student’s t-test using GraphPad Prism 8. Values were presented as mean ± SD, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Gst 4 Detoxification, supplied by Proteostasis Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/pmc13260644-259-5-9?v=Proteostasis+Therapeutics
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gst 4 detoxification - by Bioz Stars, 2026-07
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recombinant gst enpp2 protein  (Novoprotein)
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Novoprotein recombinant gst enpp2 protein
Effect of EA on anti-stress genes expression level. (A,B) EA significantly induced the expression of SOD-3::GFP. (C,D) EA significantly induced the expression of <t>GST-4::GFP.</t> (E,F) EA significantly induced the expression of HSP-16. 2::GFP after incubation at 37 °C for 30 min. (G) EA significantly activated the mRNA expression level of sod-3, gst-4 and hsp-16.2. The images were analyzed with ImageJ software and numerical data were analyzed by Student’s t-test using GraphPad Prism 8. Values were presented as mean ± SD, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Recombinant Gst Enpp2 Protein, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/pmc13197829-122-1-12?v=Novoprotein
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recombinant gst enpp2 protein - by Bioz Stars, 2026-07
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anti gst antibody  (Yeasen Biotechnology)
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Yeasen Biotechnology anti gst antibody
Effect of EA on anti-stress genes expression level. (A,B) EA significantly induced the expression of SOD-3::GFP. (C,D) EA significantly induced the expression of <t>GST-4::GFP.</t> (E,F) EA significantly induced the expression of HSP-16. 2::GFP after incubation at 37 °C for 30 min. (G) EA significantly activated the mRNA expression level of sod-3, gst-4 and hsp-16.2. The images were analyzed with ImageJ software and numerical data were analyzed by Student’s t-test using GraphPad Prism 8. Values were presented as mean ± SD, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Anti Gst Antibody, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst/pm42151485-369-41-44?v=Yeasen+Biotechnology
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anti gst antibody - by Bioz Stars, 2026-07
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Image Search Results


Effects of solubility tags and stability mutations on NcEGT1 expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 kDa), GST-NcEGT1 (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.

Journal: Synthetic and Systems Biotechnology

Article Title: Metabolic engineering of Saccharomyces cerevisiae for co-production of ergothioneine, salidroside and gadusol

doi: 10.1016/j.synbio.2026.04.010

Figure Lengend Snippet: Effects of solubility tags and stability mutations on NcEGT1 expression and EGT synthesis. (A) Expression analysis of recombinant proteins NcEGT1 and TrEGT1 in S. cerevisiae. Coomassie Blue-stained SDS-PAGE gel showing total protein extracts from different yeast strains: the parental strain (SC), strain expressing the wild-type Ncegt1 and Tregt2 genes (WT-EGT) and strain E1 expressing the codon-optimized Ncegt1 and Tregt2 genes (CO-EGT). Equal amounts of total protein were loaded. M: protein molecular weight marker. The arrowheads indicate the expected positions of NcEGT1 (≈99 kDa) and TrEGT2 (≈53 kDa). +: soluble crude extract. -: insoluble crude extract. (B) Soluble expression analysis of fusion proteins with different solubility tags. MBP-NcEGT1 (≈139.9 kDa), GST-NcEGT1 (≈125 kDa), NusA-NcEGT1(≈154.4 kDa), TrxA-NcEGT1(≈111.3 kDa) (C) Effect of different solubility tags on EGT production. (D) Effect of single-point mutations on EGT production. (E) Spatial distribution of key stability-related mutation sites in NcEGT1. The structure predicted by AlphaFold2 shows mutation sites used in double-mutant construction: A458P (pink, optimal) and five high-effect sites (blue), each combined with A458P. (F) Effect of double-point mutations on EGT production. All data presented as mean ± S.D. from three (n = 3) biological independent replicates.

Article Snippet: Among the constructed variants, a 33% increase in EGT production was observed in mutant E26 (GST-NcEGT1 A458P ) over that of E18 (GST-NcEGT1) ( D).

Techniques: Solubility, Expressing, Recombinant, Staining, SDS Page, Molecular Weight, Marker, Mutagenesis

Effect of EA on anti-stress genes expression level. (A,B) EA significantly induced the expression of SOD-3::GFP. (C,D) EA significantly induced the expression of GST-4::GFP. (E,F) EA significantly induced the expression of HSP-16. 2::GFP after incubation at 37 °C for 30 min. (G) EA significantly activated the mRNA expression level of sod-3, gst-4 and hsp-16.2. The images were analyzed with ImageJ software and numerical data were analyzed by Student’s t-test using GraphPad Prism 8. Values were presented as mean ± SD, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Frontiers in Aging

Article Title: The lifespan and healthspan extending effects of ellagic acid in Caenorhabditis elegans require an intact insulin/IGF-1 signaling pathway

doi: 10.3389/fragi.2026.1811330

Figure Lengend Snippet: Effect of EA on anti-stress genes expression level. (A,B) EA significantly induced the expression of SOD-3::GFP. (C,D) EA significantly induced the expression of GST-4::GFP. (E,F) EA significantly induced the expression of HSP-16. 2::GFP after incubation at 37 °C for 30 min. (G) EA significantly activated the mRNA expression level of sod-3, gst-4 and hsp-16.2. The images were analyzed with ImageJ software and numerical data were analyzed by Student’s t-test using GraphPad Prism 8. Values were presented as mean ± SD, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: For example, sod-3 (oxidative stress), gst-4 (detoxification), and hsp-16.2 (proteostasis) were all upregulated, consistent with our reporter assays.

Techniques: Expressing, Incubation, Software